THANKS, AND GOOD LUCK TOMORROW!

Dear T53 and T59 students

Thank you all for a good term. Working with you has been a pleasure; you are the most dynamic, enthusiastic, and fun BIOL335 groups that I've ever seen!

Good luck tomorrow and on all your other exams

Cheers

Pam

Q&As

Please check the link to our answers to practice questions regularly...I am posting Q&As that I got by email.

My email and other info

If you'd prefer to email me rather than posting a comment:

kalas[at]zoology.ubc.ca

Please note that I'll share your questions (and their answers) with the rest of the class (anonymously). They will be posted on the "our answers to practice questions..." site (see link on the right).

Extra tutorials:

Thursday, 9-11 am room 5458 in biosciences from 2449 go 2 floors up, down the corridor, 5th or 6th door on your left).

Friday 9-11 am, room 5458 (see directions above)

QUESTIONS ABOUT DR GRIGLIATTI'S SECTION

Please post here your questions regarding Tom G.'s section.

The least you need to know:

- eukaryotic genes: their elements and how they are regulated (think about everything that needs to happen in order for a eukaryotic gene to be transcribed in a given cell)

- the conept of "reporter gene" and how to use a reporter gene to test the activity of a cis-regulatory element (or to define it)

- how to clone a gene based on positional information

- how to clone a gene based on sequence homology

- when and how to use a Southern blot, a northern blot, chromosome walking, RT-PCR, footprint analysis (and how to interpret results)

- how to sequence an entire genome (steps involved)

- how to assemble a contig using molecular markers

- what a microarray is

- transposable elements: generalities, as well as P element's hybrid dysgenesis (in general) and how to use P elements to add a piece of DNA to a fly's genome, and how to exploit them to clone a gene

- transposable elements in a cross (like the question we did with corn and the Ac and Ds elements)

- types of transposable elements (very general-class I and class II)

Happy studying! 

 

QUESTIONS ABOUT DR. HAUGHN SECTION

Please post here all your questions about the last section of the course (cell cycle, cancer and development).

The least you need to be able to explain to yourself:

- how do you go from one cell to many cells that look different, act differently and work together in a coordinated way;

- the connection between gene expression, regulation of gene expression, differentiation and development;

- a few examples of types of proteins (gene products) that are involved in development (e.g. TF, signalling proteins, etc)

- a few examples of how the action of a gene, or that of a protein, can be regulated (transcriptional level, translational level, post-translational....)

- how you can get a cell to grow out of control (what "things gone wrong" may cause this)

- what type of mutations can affect cell cycle

- how to show that DNA element X( e.g. a module of an enhancer) is necessary and sufficient to confer a particular spatio-temporal expression pattern to your gene of interest;